What is Eye, Ear, Nose swab aerobic culture test?

A culture test is performed in a laboratory to grow disease-causing microorganisms and identify them. An aerobic culture test specifically looks for aerobic bacteria - bacteria that require oxygen for their growth and survival. Aerobic bacteria can grow anywhere on the exposed surface of the body. 

Though all of our body has some amount of natural flora (useful bacteria), an imbalance in the flora or growth of specific microbes may lead to infection. This test is performed to detect the causative organisms for infectious diseases related to eyes, ears and nose.

Some of the common infectious aerobic bacteria that affect these regions include Staphylococci and Streptococci.

  1. Why is an Eye, Ear, Nose swab aerobic culture test performed?
  2. How do you prepare for an Eye, Ear, Nose swab aerobic culture test?
  3. How is an Eye, Ear, Nose swab aerobic culture test performed?
  4. What do Eye, Ear, Nose swab aerobic culture test results mean?

Your doctor will order an aerobic culture test when he/she suspects that you have an infection of the eyes, ears or nose. Symptoms that are indicative of an infection include the following:

  • Eye:

    • Pain or redness inside or around the eye (e.g., margins, eyelids and white portion of the eye called sclera)
    • Sticky yellow discharge from the eye
  • Ear:
    • Pain inside and around the ear (Read more: Ear pain causes)
    • Fever
    • Pus discharge from the ear
    • The following symptoms are seen on close examination:
      • Redness inside and around the ear
      • Collection of fluid behind the eardrum
  • Nose: The symptoms are most often related to sinuses. A sinus is an empty space in the head that is connected to the nose.

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A lab technician will give you all the information regarding the test. He/she will also look for redness, pus discharge and pain based on the type of culture is to be done (eye, ear or nose). Talk to your doctor if you have any concerns about the test. Also, tell your physician if you are taking any antibiotics or other medicine.

A specimen for culture test is collected in the following manner:

  • Eye:

    • A lab technician will first clean the excess of dried discharge around your eye with a cotton or gauze piece dipped in normal saline.
    • He/she will draw back your lower eyelid and gently rub a sterile swab over the red portion of the eyelid.
    • The swab will be immediately transferred to a special bag called culturette sleeve and will be sent to the laboratory at room temperature.
  • Ear:
    • A nurse or lab technician will use a clean the excess of discharge and other dried-up debris inside your ear with the help of a gauze piece soaked in normal saline.
    • He/she will then use a swab or syringe to collect a sample of the discharge in the ear.
    • The technician will place the swab inside the ear and roll it gently to get it absorbed.
    • The swab will be kept in the culturette sleeve and will immediately be sent to the laboratory for analysis. 
  • Nasal swab:
    • A lab technician will ask you tilt your head slightly back to ease the collection of a nasal swab.
    • He/she will gently place a swab inside one of your nostrils, close to the midline wall and floor inside the nose and will gently rotate the swab for a few seconds while keeping it near the walls of the nostrils. 
    • Once the sample is collected, the technician will place the swab in a culturette sleeve and send it to the laboratory as early as possible at room temperature.

The entire procedure of the test (from specimen collection to obtaining results) takes seven to 14 days.

There are no side effects or risks associated with the eye, ear or nasal swab culture.

Normal results: 

A low count of bacteria such as Staphylococcus epidermidis or Lactobacillus species in an eye culture is considered a normal result as these are the local flora in the eyes. Similarly, local flora of the ear and nose found in their respective cultures are considered a normal result.

Abnormal results: 

Abnormal results are written as positive for the growth of infectious aerobic bacteria. 

It indicates an aerobic bacterial infection in the concerned area.

Abnormal results are obtained in the following infections:

Disclaimer: All results must be clinically correlated with the patient’s complaints to make a complete and accurate diagnosis. The above information is provided from a purely educational point of view and is in no way a substitute for medical advice by a qualified doctor.

References

  1. University of California Museum of Paleontology [Internet]. Berkeley. California. US; Bacteria: Life History and Ecology
  2. Columbia University [Internet]. New York. US; Bacterial Classification, Structure and Function
  3. MedlinePlus Medical Encyclopedia [Internet]. US National Library of Medicine. Bethesda. Maryland. USA; Ear drainage culture
  4. Sharma S. Diagnosis of infectious diseases of the eye. Eye (Lond). 2012 Feb; 26(2): 177–184. PMID: 22094299.
  5. Centers for Disease Control and Prevention [internet]. Atlanta (GA): US Department of Health and Human Services; 2020 NHSN Eye, Ear, Nose, Throat, or Mouth (EENT) Infection Checklist
  6. Kalogjera L. Rinitis u odraslih [Rhinitis in adults]. Acta Med Croatica. 2011;65(2):181–187. PMID: 22359885.
  7. Fischbach FT. A Manual of Laboratory and Diagnostic Tests. 7th ed. 2003. Lippincott Williams & Wilkins Publishers. Pp:326,327. Immunodiagnostic Studies.
  8. Albany Medical Center [Internet]. Albany. New York. US; Eye, Ear, Nose, Throat (ENT) Specimens
  9. Washington State Department of Health [Internet]. Washington D.C. US; Microbiology Laboratory Test Menu
  10. The National Academy of Sciences [Internet]. Washington D.C. United states; Types of Microbes
  11. Lagier Jean-Christophe, et al. Current and Past Strategies for Bacterial Culture in Clinical Microbiology. Clin Microbiol Rev 28:208-236.
  12. Merck Manual Professional Version [Internet]. Kenilworth (NJ): Merck & Co. Inc.; c2019. Culture
  13. Merck Manual Professional Version [Internet]. Kenilworth (NJ): Merck & Co. Inc.; c2019. Overview of Bacteria
  14. US Food and Drug Administration (FDA) [internet]. Maryland. US; Combating Antibiotic Resistance
  15. National Cancer Institute [Internet]. Bethesda (MD): U.S. Department of Health and Human Services; NCI Dictionary of Cancer Terms
  16. University of Rochester Medical Center [Internet]. Rochester (NY): University of Rochester Medical Center; Microbiology
  17. UW Health: American Family Children's Hospital [Internet]. Madison (WI): University of Wisconsin Hospitals and Clinics Authority; Using Antibiotics Wisely
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