What is Rotavirus Antigen test?

A rotavirus antigen test determines the presence of rotavirus, the causative agent of a type of gastroenteritis, in your stool.

Rotavirus is a contagious virus that most commonly affects unvaccinated children between the age of three to 35 months, and causes severe diarrhoea. Though, the virus can also affect adults. The infection spreads more commonly in winter and spring.

Once infected, the person starts eliminating the virus in their stool, which can then spread to healthy people through environmental contamination. A person can get infected in the following ways:

  • By eating with unwashed, contaminated hands
  • By touching a contaminated surface or an object and then putting fingers in the mouth
  • By consuming contaminated food
  1. Why is a Rotavirus Antigen test performed?
  2. How do you prepare for a Rotavirus Antigen test?
  3. How is a Rotavirus Antigen test performed?
  4. What do Rotavirus Antigen test results mean?

Your doctor may order a rotavirus antigen test if you show symptoms of rotavirus infection. Symptoms usually show up after two days of exposure to the virus. Vomiting and diarrhoea are the most common symptoms. Other symptoms include: 

Diarrhoea may lead to dehydration in some cases, which manifests as:

  • Dizziness when you stand up
  • Dry mouth and throat
  • Less urination than usual

In children, the symptoms related to dehydration may be as follows:

  • Thirst 
  • Being cranky and restless
  • Sunken eyes
  • Dry mouth and tongue
  • Dry skin
  • Sunken soft spot on the head 
  • Less urination
  • Dry diaper for hours
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You do not need any preparation for this test. Inform your doctor if you are taking any prescribed, non-prescribed and illicit medicines or vitamins and supplements.

A stool sample is collected for rotavirus detection. Your healthcare practitioner will give a plastic container for the collection of the sample. The sample can be collected in the following way:

  • Label the container with your name, date of birth and the date of collection of sample. 
  • Place a potty seat or an empty plastic container in the toilet seat. You can even wrap a clean newspaper or plastic wrap on the rim of the toilet seat. 
  • The stool should not touch the inner area of the toilet. 
  • Now, using a spoon or spatula, collect the stool in the container and close the lid. 
  • The stool sample should be filled to one-third of the container. 
  • Put the material used for collection in a plastic bag and discard it in the dustbin. 
  • Rinse your hands properly with soap and warm water after the test.

The stool sample should be collected when the person has diarrhoea. If the test is ordered for babies, attach a plastic wrap to a diaper and position it properly to avoid getting urine in the stool sample.

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Normal results:

A normal result means that no rotavirus is detected in your stools.

Abnormal results:

An abnormal result is reported as positive for the rotavirus antigen. It indicates that you may have a rotavirus infection.

Disclaimer: All results must be clinically correlated with the patient’s complaints to make a complete and accurate diagnosis. The above information is provided from a purely educational point of view and is in no way a substitute for medical advice by a qualified doctor.

References

  1. Haines CF, Sears CL. Infectious enteritis and proctocolitis. In: Feldman M, Friedman LS, Brandt LJ, eds. Sleisenger and Fordtran's Gastrointestinal and Liver Disease. 10th ed. Philadelphia, PA: Elsevier Saunders; 2016:chap 110.
  2. Bass DM. Rotaviruses, calciviruses, and astroviruses. In: Kliegman RM, Stanton BF, St. Geme JW, Schor NF, eds. Nelson Textbook of Pediatrics. 20th ed. Philadelphia, PA: Elsevier; 2016:chap 265, 340.
  3. Semrad CE. Approach to the patient with diarrhea and malabsorption. In: Goldman L, Schafer AI, eds. Goldman-Cecil Medicine. 25th ed. Philadelphia, PA: Elsevier Saunders; 2016:chap 140.
  4. Center for Disease Control and Prevention [internet], Atlanta (GA): US Department of Health and Human Services; Rotavirus
  5. University of Rochester Medical Center [Internet]. Rochester (NY): University of Rochester Medical Center; Rotavirus (Stool)
  6. National Health Service [internet]. UK; How should I collect and store a poo (stool) sample?
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