Lab test

Laboratory tests are important diagnostic, monitoring and screening tools for healthcare practitioners. They help doctors detect health conditions, plan an appropriate treatment for the patient and monitor treatment success. Some tests are also done as a part of routine health check-ups such as ECG, cholesterol test and blood sugar test.

In research labs, medical tests help scientists to find out the pathophysiology of a disease - which refers to the way in which a disease affects the body functions. This is especially helpful to study and find treatment for unknown or new diseases and infections.

Depending on the condition to be checked, lab tests are of different types and may need body fluids or tissues as specimens. Results are interpreted on the basis of a reference range. 

A reference range for a test indicates the normal results for that test. It is set in a lab as per the test results from a majority of healthy people who got that particular test done in the lab. 

Here are the different types of lab tests along with ways to interpret your test results.

  1. Types of lab tests
  2. Preparing for a test
  3. Questions to ask your doctor before the test
  4. Understanding test results
  5. Lab tests price

Types of lab tests

There are different types of lab tests and your doctor may ask you to get more than one depending on your condition and symptoms. However, tests can be broadly divided into the following types:

  1. Body fluid analysis
  2. Genetic testing
  3. Monitoring body functions
  4. Imaging tests
  5. Biopsy
  6. Cytology tests
  7. Endoscopy
  8. Microbiology tests

Body fluid analysis

Most tests involve body fluid analysis. Some fluids that are analysed include blood, urine, sputum, sweat, synovial fluid (fluid in joints) and interstitial fluids (fluids present in spaces around body cells) such as cerebrospinal fluid (CSF) in your brain and spinal cord, pleural fluid (fluid present between the chest cavity), ascitic fluid (present in your abdomen). 

A blood sample is obtained through a finger prick, venipuncture or heel prick method and to collect a urine sample, the patient is given a sterile container. Other fluids are commonly obtained through inserting a fine needle into the concerned body cavity, for example, a spinal tap is done to withdraw a CSF sample. In this procedure, a hollow needle is put in between two vertebrae to withdraw the cerebrospinal fluid.

Body fluids are usually analysed for pathological tests such as to look for abnormalities in the components of these fluids, presence of cancerous cells, infectious microbes or antibodies to specific microbes (serological tests). Blood and urine are also analysed to monitor the therapeutic levels of certain drugs in the patient’s body.

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Genetic testing

Genetic testing helps check for the presence of abnormalities in the DNA, chromosomes or proteins in an individual. It helps to check if a person has a genetic condition or is at risk of developing one. This test can be done on body fluids or tissues and looks for missing genes, changes in genetic sequences and/or production of certain proteins to check the activity of a stretch of DNA. Genetic testing procedures like amniocentesis and chorionic villus sampling are specially done during pregnancy to look for birth defects in the child.

Additionally, certain types of breast cancer and colorectal cancer can be detected during the early stages through genetic testing. It also helps monitor and plan the treatment of these cancers.

Monitoring body functions

These tests check how well a particular organ in your body is functioning and include tests like ECG and EEG, which measure the functioning of your heart and brain respectively along with tests for monitoring liver, kidney and lung function.

(Read more: Liver function test procedure)

Imaging tests

Imaging tests are non-invasive procedures that provide pictures of the internal structures of your body. Common imaging tests include:

X-rays: In this test, the patient is asked to stand, sit or lie in front of a machine that emits x-rays. X-rays are invisible and intangible radiations which can pass through the body. However, as they travel through the human body, some amount of x-rays are absorbed by dense body tissues, like bones whereas soft tissues like organs do not absorb any. Whatever remains is then captured by a detector placed on the other side of the patient’s body, which then helps produce images of the part of the body being tested. So while bones would appear dark grey in an X-ray film, lungs appear black.

X-rays can be used to see both bones and soft tissues. An x-ray may be done to look for tooth and bone problems or injuries, lung problems, breast cancer (x-ray mammogram) and to check for the presence of metal objects stuck in the body like ingested coins. X-rays are also used to guide surgical procedures like to check for the proper placement of metal implants in joints or bones and in coronary angioplasty to guide the balloon into the right blood vessel.

Ultrasounds: An ultrasound test uses a tiny probe to send sound waves into the concerned area of the patient’s body. These waves hit body tissues and bounce back to the probe, which then transfers the information to a computer to make an image of the tissue being tested. The images can be seen in real-time and can also be printed for later evaluation. Ultrasound tests are much safer than x-rays since they don’t involve any radiation exposure. Also, these tests can help visualise real-time changes in the body such as the movement of the fetus in the womb and blood flow through an artery (Doppler ultrasound). 

Depending on the condition that needs to be checked, the probe is either moved over the patient’s body (external ultrasound) or inserted into their body (internal ultrasound). An endoscopy ultrasound is one where the ultrasound probe is attached on the tip of a long rod (an endoscope) and is passed into the patient’s body to take images. 

Ultrasound can be used to check for problems with the structure or function of an organ. Doctors usually order an ultrasound for conditions like fibroids, stones, cysts, tumours and lumps in the body. High intensity focussed ultrasounds are a type of therapeutic ultrasounds in which very high-intensity sound waves are used to generate heat and clear blood clots, treat uterine fibroids or tumours in the body. 

MRI: Magnetic resonance imaging or an MRI scan uses a combination of a magnetic field and radio waves to generate pictures of the internal structures of the body. An MRI machine is like a tunnel, the patient lies on the MRI table which then slides into the tunnel. During the scan, the machine usually makes a lot of noise and the patient is given headphones to mute the sound. The technician stays in an adjoining room but the patient can contact him/her through an intercom.  

For claustrophobic patients, some places also offer a reclined open and a standing open MRI, where the patient either sits or stands in the MRI machine. MRI scans are used to check any body part including bones, joints, and soft tissues. This imaging test can also help check the brain function in real-time.

CT scan: A Computed Tomography (CT) scan also uses x-ray radiations to produce images of internal structures of the body. However, a CT scan machine has a circular scanner that rotates around the patient’s body while emitting and collecting x-rays and creating tomographic images (tiny slice images) of the patient’s body. A computer attached to the machine then collects all these slices to create a complete 3-D image. 

Images obtained from a CT scan are hence much more detailed than a regular x-ray. Also, CT scans can be done to visualise any part of the body including soft tissues. Some of the things that a CT scan helps look for include injuries, tumours, blood flow, stroke and conditions like pneumonia and emphysema

Contrast vs non-contrast imaging tests: A contrast dye is sometimes injected or given orally to patients before undergoing an imaging test. These dyes are taken up by tissues that need to be examined, which makes it appear a bit different than the surrounding tissues and is hence more clearly visible. 

After the test, the dye slowly gets flushed out of the body.

PET scan: A positron emission tomography or PET scan involves the administration of a radioactive tracer to the patient either through injection, inhalation or oral route. This tracer is absorbed by the tissues, leading them to emit gamma rays inside your body. A PET scan machine, which looks a lot like a CT scan machine, picks up the radiations emitted by these tissues and present a complete image of the concerned tissue on the computer screen. A PET scan is much more sensitive than an MRI and CT scan as it can identify problems while they are still at the cellular level. PET scan is most commonly done to check for cancer screening and diagnosis and to monitor the treatment of cancer. It is also used to diagnose conditions like Parkinson’s and epilepsy and coronary artery disease


A biopsy is a minimally invasive procedure in which a small sample of tissue is cut from the body and examined under a microscope. The tissue sample can be obtained by punching a tiny hole in the tissue like a skin biopsy, through endoscopy or a fine needle. A biopsy helps in both diagnoses and monitoring diseases such as cancer, inflammatory conditions, infections and skin diseases

Cytology tests

Cytology tests are a type of pathology tests that are done to study cell samples, either a single cell or a group of cells. These tests can be done on any of the body fluids or tissue samples. Tissue samples for cytology tests are obtained by scraping or brushing or fine-needle aspiration, wherein a very thin needle is used to extract a small amount of body fluids for testing.


Just like imaging procedures, an endoscopy is done to examine the internal structures of the body. In this procedure, a flexible rod with a camera and a light attached on its tip is inserted into the patient’s body through any of the natural openings like nose, urethra, mouth or vagina. As the rod goes in, the technician can see the inside of the patient’s body on an attached screen. Sometimes a tiny excision is made to insert an endoscope like in the case of a laparoscopy (to visualise abdominal cavity) and thoracoscopy (to visualise lungs and the outer covering of lungs).

Microbiology tests

Microbiological tests are done along with pathology tests to identify pathogenic microbes and to find out what antibiotics would work best against the identified microbes. Microbiological tests are done on any body fluid including blood, saliva, urine and CSF or on swabs taken from tissues. The samples are spread on a special culture plate and are incubated at a specific temperature to allow microbial growth. It takes 24 to 48 hours for bacteria to grow and about 5-7 days for fungal growth. The isolated microbes are then stained and viewed under a microscope to identify them. To find out the antibiotic that is most effective against the isolated microbe, the microbe is replated in a Petri plate that either contains the said antibiotic or has antibiotic discs placed over it. The antibiotic that suppresses the growth of the microbe is chosen for treatment. 

Preparing for a test

Most tests require a certain amount of preparation on the patient’s part. While ordering the test, doctors usually tell the patient about all of these preparations. Make sure you follow your doctor's instructions before you go for a test as these ensure that the test results are accurate. Generally, it is advised that a patient should inform the doctor if they are taking any medications, both prescribed and non-prescribed, or health supplements, as a number of medications interfere with the results of lab tests. Here are some other preparations that are most commonly needed before going for a lab test:

  • Fasting may be needed for some tests. Usually, the patient has to refrain eating anything for about 8 to 12 hours before a test; however, they are allowed to drink water.
  • For some tests, patients are asked to drink a lot of water before they come in for the test or to avoid certain foods, coffee, tea or smoking.
  • For semen analysis, samples are collected within 3 to 5 days of abstinence and would be asked to empty their bladder before ejaculating.
  • Before a pap smear, women are told to avoid bathing in a tub or douching at least 24 hours before the test and to not have sex or use a vaginal cream a day or two before the test.
  • For procedures like a biopsy and endoscopy that involve administration of anesthetics, patients are asked to bring someone along to help them go back home after the test.

(Consult physician online)

Questions to ask your doctor before the test

Normally, doctors explain the whole test procedure to their patients before they conduct the test, especially if the patient is particularly anxious about anything such as needles or closed spaces (for MRI). However, if you can't shake the worry and fear of the test, it is best to talk to the doctor to know more. Here are some questions you can ask your doctor before going for a test:

  • What is the purpose and procedure of the test?
  • What are the possible risks and side effects of the test?
  • Do I have to be concerned about something before, during or after the test?
  • How soon will I get the test results and what would the results show?
  • Is there something that may affect the test results?

If you are still anxious, you can try to breathing or relaxing techniques before the test or tell your doctor about it.

Understanding test results

Most test results are given in the form of a table mentioning the reference range and the results obtained. The reference range is the standard results that the laboratory considers to be normal. It is important to note that this reference range varies from lab to lab and a result that may fall in the normal range at one lab may be considered abnormal in another lab. 

If your results fall in the normal range, it means you are in good health and don’t have the condition that was being tested. However, results above or below the reference range are said to be abnormal and that indicates that you may have the condition being tested.

A false positive or false negative results may sometimes be obtained in a test. If you otherwise seem healthy, your doctor may ask for further tests to confirm the diagnosis. 

An uncertain test result means the test could not rule out the disease. In this case, you’ll have to get more tests done as well.

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Lab tests price

The cost of a lab test would vary depending on various factors including the lab you are getting tested at, the type of test, and the sensitivity of the test (how precise the test results are). So while some blood tests start from Rs. 100 (in some government hospitals this begins at Rs. 50) electrophoresis tests may go above Rs. 7,000 in private labs. Microbiology tests, especially culture tests, are a bit costly. They begin anywhere around Rs. 500 and may go up and above Rs. 10,000. Again, in some government labs, microbiology culture tests may cost as low as Rs. 50-250.

Radiology tests such as an MRI may cost you around Rs. 2,500-3,000 in a government hospital and may range from Rs. 3,000 to above Rs. 20,000 for MRIs of specific body parts in private labs. Similarly, an X-ray may cost you anywhere around Rs. 250 to above Rs. 5,000. 

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